Schuler
Fakultäten » Medizinische Fakultät » Biochemisches Institut » Prof. Dr. Ben Schuler » Schuler
Completed research project
| Title / Titel | Single Molecule Protein Folding: Investigating Complex Folding and Misfolding Mechanisms with Multiparameter Fluorescence Spectroscopy |
||||
|---|---|---|---|---|---|
| Abstract (PDF, 14 KB) | |||||
| Summary / Zusammenfassung | In this collaborative project we investigate the competition between protein folding and misfolding using components of biochemistry, spectroscopy and theoretical biophysics. We combine the powerful method of single molecule multiparameter fluorescence detection with resonance energy transfer between up to three dyes, advanced strategies for fluorophore attachment and sample immobilization, parallel two-color excitation, photon detection in up to six channels and the development of theoretical methods including molecular simulations for the structural interpretation of the results. This will not only contribute to understanding the causes of the potentially harmful process of protein misfolding, but also extend the available methods for the single molecule analysis of complex systems. | ||||
| Project leadership and contacts / Projektleitung und Kontakte |
|
||||
| Funding source(s) / Unterstützt durch |
Foundation VolkswagenStiftung, Hannover, Germany |
||||
| In collaboration with / In Zusammenarbeit mit |
|
||||
| Duration of Project / Projektdauer | Jul 2003 to Dec 2007 |