Fakultäten » Medizinische Fakultät » Biochemisches Institut » Prof. Dr. Peter Sonderegger (emeritiert) » Sonderegger

Completed research project

Title / Titel The calsyntenin family of postsynaptic membrane proteins
PDF Abstract (PDF, 14 KB)
Summary / Zusammenfassung We have discovered a novel family of postsynaptic membrane proteins, the calsyntenins. The currently best characterized member of the calsyntenin family is calsyntenin-1. Calsyntenin-1 is a transmembrane protein located in the postsynaptic membrane of CNS synapses. It contains two cadherin-line domains at the N-terminus of its extracellular moiety and a highly acidic cytoplasmic domain. Proteolytic processing in the extracellular moiety results in the internalization of the transmembrane stump. Internalization after proteolytic cleavage in the extracellular domain, thus, modulates the presence of calsyntenin-1, and possibly other proteins, in the postsynaptic membrane. Therefore, we speculate that calsyntenin-1 may regulate the molecular composition of the postsynaptic and, thus, control synaptic changes (plasticity) underlying learning and memory functions.
We also identified two novel postsynaptic membrane proteins that are highly similar to calsyntenin-1 in their extracellular parts, but vary considerably in their cytoplasmic segment. Based on their structural similarity to calsyntenin-1, we have called the novel proteins, calsyntenin-2 and -3, respectively. By immuno-electron microscopy, the novel calsyntenin family proteins were localized in the postsynaptic membrane of excitatory central nervous system (CNS) synapses. In situ hybridization analysis revealed that calsyntenin-1 was abundant in most neurons of the CNS with relatively little variation in its expression level. Calsyntenin-2 and -3 expressions varied much more with highest levels in GABAergic neurons. Based on their distinct expression patterns and the differences in their cytoplasmic segments, we suggest a cell-type specific functional role for the three calsyntenins in excitatory synaptic transmission.
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Publications / Publikationen Vogt L., Schrimpf S.P., Meskenaite V., Frischknecht R., Kinter J., Leone D.P., Ziegler U., and Sonderegger P. Calsyntenin-1, a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain, Molec. Cell. Neurosci. 17: 151-166 (2001)

Hintsch G., Zurlinden A., Meskenaite, V., Steuble M., Fink-Widmer K., Kinter J., and Sonderegger P. The calsyntenins - a family of postsynaptic membrane proteins with distinct neuronal expression patterns, Molec. Cell. Neurosci. 21: 393-409 (2002)

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Keywords / Suchbegriffe Calsyntenin-1, calsyntenin-2, calsyntenin-3, postsynaptic membrane, synaptic plasticity, memory and learning, LTP, long-term potentiation
Project leadership and contacts /
Projektleitung und Kontakte
Prof. P. Sonderegger (Project Leader)
Other links to external web pages
Funding source(s) /
Unterstützt durch
SNF (Personen- und Projektförderung), SNF (Programm NFS/NCCR), Private Sector (e.g. Industry)
University of Zürich, Neurotune AG
In collaboration with /
In Zusammenarbeit mit
B. Ledermann, Universität Zürich
Th. Rülicke, Universität Zürich
D. Wolfer, Universität Zürich
P. Streit, University of Zürich
Duration of Project / Projektdauer Sep 2002 to Dec 2006