Zimmermann
Fakultäten » Medizinische Fakultät » Molekulare Diagnostik, Labor für » Prof. Dr. Dieter Zimmermann » Zimmermann
Current research project
| Title / Titel | Translational research projects (Diagnostic microsatellite analysis / Detection of translocations in lymphomas and soft tissue tumors / Detection of bacterial and viral infections / Gene mutation and methylation analysis) | ||
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| Abstract (PDF, 14 KB) | |||
| Summary / Zusammenfassung | One of the current research projects focuses on the improvement of the detection of gene methylation status in fresh and formalin-fixed tissues. Special emphasis is set on improving reproducibility and quantitative aspects in order to fulfill the strict quality requirements for diagnostic applications. Furthermore, we are working on improving the detection of specific translocations using different PCR-techniques. Special fields of interest are lymphomas and soft tissue tumor diagnostics on fresh and formalin fixed-tissues. Finally, we regularly establish sensitive gene mutation analyses required for prognosis or for prediction of target therapies in cancer. Our routine diagnostics in the laboratory of molecular diagnostics currently includes: - Clonality assessment of lymphoproliferative diseases with Southern blot and PCR - Detection of t(14;18), t(11;14) and t(11;18) translocations in lymphoma with standard, RT- and long-distance PCR - Detection of t(X;18) in synovial sarcoma, t(11;22) and t(21;22) in Ewing sarcoma, t(12;16) and t(12;22) in myxoid liposarcoma and t(12;22) in clear cell sarcoma in fresh-frozen and formalin-fixed tissues using RT-PCR - Detection of loss of heterozygosity (LOH) of specific microsatellite-markers in bioptic and cytological samples of bladder carcinoma patients using PCR and capillary electrophoresis - Detection of loss of heterozygosity (LOH) of specific microsatellite-markers for the diagnosis and prognosis of oligodendroglioma - Detection of microsatellite instability in colon carcinoma - Gene mutation analysis (EGFR, ERBB2, KIT, PDGFRA, KRAS, BRAF, CEBPA, CTNNB1, GNAS, HFE, PRNP, JAK2, IDH1 and 2) by PCR and direct DNA sequencing - Detection of mycobacterium tuberculosis infections in formalin-fixed tissues with PCR - Detection and typing of non-tuberculosis mycobacteria in formalin-fixed tissues with PCR and sequencing - Detection and typing of human papillomavirus infections from cytological preparations and biopsies using PCR and DNA sequencing - Detection of human herpesvirus-8 with PCR |
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| Publications / Publikationen | Leuenberger, M., Frigerio, S., Wild, P., Noetzli, F., Korol, D., Zimmermann, D.R., Gengler, C., Probst-Hensch, N.M., Moch, H. & Tinguely, M. (2010) AID protein expression in chronic lymphocytic leukemia/small lymphocytic lymphoma is associated with poor prognosis and complex genetic alterations. Modern Pathol., 23, 177-186.Weber, A., Zimmermann, D.R. & Moch (2010) KRAS-Mutationsstatus und kolorektales Karzinom. Köberle, D. & Gautschi, O. (eds). UNI-MED Science, UNI-MED Verlag AG, Bremen, 29-33.Bode-Lesniewska, B., Frigerio, S., Exner, U., Abdou, M.T., Moch, H. & Zimmermann, D.R. (2007) Relevance of translocation type in myxoid liposarcoma and identification of a novel EWSR1-DDIT3 fusion. Genes Chromosomes Cancer 46, 961-971.Petersen, I., Schewe, C., Schlüns, K., Speich, N., Schmitt, C., Bollmann, M., Sotlar, K., Dours-Zimmermann, M.T., Padberg, B.C. & Zimmermann, D.R. (2007) Inter-laboratory validation of PCR-based HPV detection in pathology specimens. Virchows Arch. 451, 701-716.Frigerio, S., Padberg, B.C., Strebel, R.T., Lenggenhager, D. M., Messthaler, A., Abdou, M.T., Moch, H. & Zimmermann, D.R. (2007) Improved detection of bladder carcinoma cells in voided urine by standardised microsatellite analysis. Int. J. Cancer 121, 329-338.Bode, B.°, Frigerio, S.°, Behnke, S., Senn, B., Odermatt, B., Zimmermann, D.R. & Moch, H. (2006) Mutations in the tyrosine kinase domain of the EGFR gene are rare in synovial sarcoma. Modern Pathol. 19, 541-547. (° equal contribution)Padberg, B.C., Bode, B. & Zimmermann, D.R. (2004) Unaufhaltsamer Schritt in die richtige Richtung: HPV-Typisierung und gynäkologische Krebsvorsorge. Schweiz. Med. Forum 4, 851-854.Albinger-Hegyi, A., Hochreutener, B., Abdou, M.T., Hegyi, I., Dours-Zimmermann, M.T., Kurrer, M.O., Heitz, Ph.U. & Zimmermann, D.R. (2002) High frequency of t(14;18)-translocation breakpoints outside of major breakpoint and minor cluster regions in follicular lymphomas – improved PCR protocols for their detection. Am. J. Pathol. 160, 823-832Padberg, B.C., Rodrigues, C. & Zimmermann, D.R. (2002) Molekularbiologischer Nachweis und Typisierung humaner Papilloma-Viren in der gynäkologischen Krebsvorsorge. Schweiz. Med. Forum 2, 1002-1005.Zimmermann, D.R., Städeli-Brodbeck, R., Ajmo, M., Dours-Zimmermann M.T.,Pfyffer, G. E. & Heitz, Ph. U. (1997) Detection of Mycobacteria - A Molecular Pathology Approach. Verh. Dtsch. Ges. Pathol. 81, 273-280.Weitere Informationen | ||
| Keywords / Suchbegriffe | lymphomas, gene rearrangement, clonality, translocation, sarcoma, EGFR, KIT, KRAS, mutation analysis, lung carcinoma, GIST, colon carcinoma, LOH analysis, oligodendroglioma, bladder carcinoma, mycobacterium tuberculosis, non-tuberculosis mycobacteria, human papillomavirus, cervix carcinoma, human herpesvirus-8 | ||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
Forschungskredit der Universität Zürich |
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| Duration of Project / Projektdauer | Jan 1990 to Dec 2015 |