Siler

Fakultäten » Medizinische Fakultät » Kinderspital Zürich: Medizinische Klinik » Immunologie, Abteilung » Prof. Dr. Reinhard Seger (emeritiert) » Siler

Completed research project

Title / Titel	Monitoring of pediatric CGD gene therapy patients
PDF	Abstract (PDF, 14 KB)
Summary / Zusammenfassung	Presently our laboratory is able to analyze blood and bone marrow samples of adult and infant CGD gene therapy patients by following assays: • FACS analysis of gp91phox • Analysis of gp91phox – p22phox assembly within the NADPH oxidase (Cytophrome B reduced minus oxidized difference spectrum) • Qualitative detection of respiratory burst activity (NBT reduction, DHR 123 oxidation, Chemiluminescence assay) • Quantitative analysis of respiratory burst activity (Cytochrome C reduction assay) • E.coli killing assay • A.fumigatus killing assay • Quantification of the percentage of gene modified cells in bone marrow stem/progenitor cells (colony assay combined with PCR based transgene detection in individual colonies of the colony assay). • NET formation assay Since 2005 we were able to monitor adult an infant patients after clinical CGD GT.
Publications / Publikationen	Ott MG, Schmidt M, Schwarzwaelder K, Stein S, Siler U, Koehl U, Glimm H, Kuhlcke K, Schilz A, Kunkel H, Naundorf S, Brinkmann A, Deichmann A, Fischer M, Ball C, Pilz I, Dunbar C, Du Y, Jenkins NA, Copeland NG, Luthi U, Hassan M, Thrasher AJ, Hoelzer D, von Kalle C, Seger R, Grez M. Correction of X-linked chronic granulomatous disease by gene therapy, augmented by insertional activation of MDS1-EVI1, PRDM16 or SETBP1. Nat Med. 2006; 12: 401-9. M.G.O., M.S., K.S., S.S. and U.S. contributed equally to this work C.v.K., R.S. and M.G. share senior authorship
Keywords / Suchbegriffe	Gene Therapy, CGD, NADPH oxidase
Project leadership and contacts / Projektleitung und Kontakte	Dr. Ulrich Siler (Project Leader) ulrich.siler@kispi.uzh.ch
Funding source(s) / Unterstützt durch	No project-specific funding
In collaboration with / In Zusammenarbeit mit	Dr. M.Grez, Georg-Speyer Haus, Frankfurt a.M, Germany Germany
Duration of Project / Projektdauer	Apr 2005 to Oct 2011