Hoop
Fakultäten » Vetsuisse-Fakultät » Veterinärbakteriologie, Institut für » Prof. Dr. Richard Hoop » Hoop
Completed research project
| Title / Titel | A new approach - the glycan-based vaccine against Campylobacter | ||||||
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| Abstract (PDF, 14 KB) | |||||||
| Summary / Zusammenfassung | Campylobacteriosis is an important food-borne human disease in developed countries. The infection source is – among others such as water and milk – poultry meat. Successful control in poultry production is still missing, mainly due to the unknown infection routes on Campylobacter in livestock production and the lack of effective post-slaughter measures. Several strategies to control Campylobacter prevalence are imposed on several stages of production, namely on the farm, in the slaughterhous and in the kitchen of the consumer. The utmost emphasis has gained the control of Campylobacter in livestock due to the scientist recommendations during recent years. These strategies during broiler production include competitive exclusion (e.g. using probiotics), genetic resistance of the host and vaccination. Vaccination of chickens against Campylobacter is based on the observation that chicken colonized with C. jejuni develop a systemic and mucosal humoral response of the IgY (IgG), IgA and IgM type. Increasing levels of antibodies result in a decreasing level of colonization, suggesting that the acquired response results in a protection against colonization. However, effective vaccination against Campylobacter has still to be developed. The use of live oral vaccines of attenuated Campylobacter strains is not recommended due to the genetic instability of this species. As an alternative strategy, live vectors to present Campylobacter proteins have been used. In this study, we suggest a novel strategy for the generation of a live vaccine that is based on the highly conserved protein glycosylation pathway of C. jejuni. N-linked protein glycosylation was recently discovered in C. jejuni and it was possible to transfer the functional pathway into the model organism Escherichia coli. This transfer made it possible to study this biosynthetic process at a molecular level: the branched heptasaccharide GIc(GaINAc)5Bac is assembled on the lipid carrier bactoprenylpyrophosphate at the cytoplasmic side of the plasma membrane, flipped to the periplasm and transferred to selected asparagine residues of different polypeptides. Glycans are highly efficient antigens for vaccination if they are presented in a high density as LPS or LOS in Gram-negative bacteria. |
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| Keywords / Suchbegriffe | glycan, vaccine, Campylobacter, broiler | ||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
Other Public Sources (e.g. Federal or Cantonal Agencies) |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | Jan 2010 to Dec 2011 |